Radioimmunoassay of estriol-16-glucuronide. [Tritium tracer techniques]
Journal Article
·
· J. Clin. Endocrinol. Metab.; (United States)
A specific radioimmunoassay of estriol-16-glucuronide has been developed, using an antiserum obtained by immunization of rabbits against estriol-16-glucuronide-BSA. The assay does not require hydrolysis, extraction and purification, but only a dilution of the crude sample. This constitutes the main advantage of the procedure. Accuracy, precision and sensitivity of the method are similar to those reported for other radioimmunoassays of estriol. Its specificity is good for the measurement of estriol-16-glucuronide in urine and in amniotic fluid, but not in serum of pregnant women, which apparently contains material interfering with the radioimmunoassay, at dilutions of less than 1/100. A significant correlation was observed between estriol-16-glucuronide and total estriol in urine during pregnancy; however, the contribution of the glucuronide to the total increases as pregnancy progresses, rising from 50% for a total of 5 mg/24 h to 85% for a total of 50 mg/24 h. The present radioimmunoassay can be used as a quick and reliable method for the measurement of urinary estriol-16-glucuronide in high risk pregnancies.
- Research Organization:
- Brussels Univ.
- OSTI ID:
- 5276723
- Journal Information:
- J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 45:5; ISSN JCEMA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Direct radioimmunoassay for estriol-16-glucuronide in urine for monitoring pregnancy and induction of ovulation. [Tritium tracer techniques]
Direct radioimmunoassay for estriol-16. cap alpha. -glucosiduronate: its use in the determination of plasma and urine levels and renal clearance of this conjugate in pregnancy
Improved radioimmunoassay of urinary estriol. [/sup 125/I tracer technique]
Journal Article
·
Mon Aug 01 00:00:00 EDT 1977
· Am. J. Obstet. Gynecol.; (United States)
·
OSTI ID:7086827
Direct radioimmunoassay for estriol-16. cap alpha. -glucosiduronate: its use in the determination of plasma and urine levels and renal clearance of this conjugate in pregnancy
Journal Article
·
Thu Jun 01 00:00:00 EDT 1978
· Am. J. Obstet. Gynecol.; (United States)
·
OSTI ID:6845307
Improved radioimmunoassay of urinary estriol. [/sup 125/I tracer technique]
Journal Article
·
Sun Dec 31 23:00:00 EST 1978
· Clin. Chem.; (United States)
·
OSTI ID:6530972
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
ESTRANES
ESTRIOL
ESTROGENS
GLUCURONIC ACID
HORMONES
HYDROGEN ISOTOPES
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PREGNANCY
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
ESTRANES
ESTRIOL
ESTROGENS
GLUCURONIC ACID
HORMONES
HYDROGEN ISOTOPES
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PREGNANCY
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES