Direct radioimmunoassay for estriol-16. cap alpha. -glucosiduronate: its use in the determination of plasma and urine levels and renal clearance of this conjugate in pregnancy

Journal Article · Thu Jun 01 04:00:00 EDT 1978 · Am. J. Obstet. Gynecol.; (United States)

DOI:https://doi.org/10.1016/0002-9378(78)90596-3· OSTI ID:6845307

Wright, K; Collins, D C; Virkler, M; Musey, P I; Dale, E; Preedy, J R.K.

A direct radioimmunoassay which does not require hydrolysis or chromatography has been developed for estriol-16..cap alpha..-glucosiduronate in pregnancy plasma and urine. The antigen used in the development of this antiserum was prepared by joining the carboxylic acid group of estriol-16..cap alpha..-glucosiduronate covalently to the epsilon-amino group of the lysine residues in bovine serum albumin. Results indicate that the direct radioimmunoassay yields levels of estriol-16..cap alpha..-glucosiduronate in late pregnancy urine that are comparable to those obtained by more elaborate procedures involving chromatographic separation of the estriol conjugates, followed by enzymic hydrolysis and measurement of the freed estriol. In addition, good correlation was found between the total estrogen values in urine obtained by currently used chemical procedures and urinary levels of estriol-16..cap alpha..-glucosiduronate. The renal clearance of estriol-16..cap alpha..-glucosiduronate was determined in eight normal women during the third trimester, and the mean value +-S.D. was 404 +- 81 ml. per minute (range, 248 to 494). This method is suitable for the evaluation of variations in the plasma and urine levels of estriol-16..cap alpha..-glucosiduronate in pregnancy and offers significant advantages over the presently used chemical methods for monitoring fetal well-being.

• Research Organization: Emory Univ., Atlanta
• OSTI ID: 6845307
• Journal Information: Am. J. Obstet. Gynecol.; (United States), Journal Name: Am. J. Obstet. Gynecol.; (United States) Vol. 131:3; ISSN AJOGA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
CLEARANCE
ESTROGENS
EXCRETION
FEMALES
HORMONES
IMMUNE SERUMS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MAN
PREGNANCY
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RENAL CLEARANCE
STEROID HORMONES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
VERTEBRATES
WASTES
WOMEN