Direct radioimmunoassay for estriol-16-glucuronide in urine for monitoring pregnancy and induction of ovulation. [Tritium tracer techniques]

Journal Article · Mon Aug 01 04:00:00 EDT 1977 · Am. J. Obstet. Gynecol.; (United States)

DOI:https://doi.org/10.1016/0002-9378(77)90723-2· OSTI ID:7086827

Haning, Jr, R V; Satin, K P; Lynskey, M T; Levin, R M; Speroff, L

Antibodies to estriol-16..cap alpha..-(..beta..-D-glucuronide) were raised in sheep with the use of keyhole limpet hemocyanin and bovine serum albumin conjugates of estriol-16..cap alpha..-(..beta..-D-glucuronide). A simple, rapid method is presented for direct radioimmunoassay of estriol-16..cap alpha..-(..beta..-D-glucuronide) in urine with dextran-coated charcoal used for separation of free from bound and deionized water used for dilutions. The method is thrifty in its use of reagents. The assay has been evaluated in the pregnancy range, and the sensitivity has been extended into the range necessary for monitoring induction of ovulation with pergonal.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 7086827
• Journal Information: Am. J. Obstet. Gynecol.; (United States), Journal Name: Am. J. Obstet. Gynecol.; (United States) Vol. 128:7; ISSN AJOGA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY FLUIDS
DOMESTIC ANIMALS
ESTRANES
ESTRIOL
ESTROGENS
HORMONES
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OVULATION
PREGNANCY
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RUMINANTS
SHEEP
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM
URINE
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES