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Title: Hormonal monitoring of early pregnancy by a direct radioimmunoassay of steroid glucuronides in first morning urine

Abstract

The usefulness of the direct 4-hour radioimmunoassay of estriol-16-glucuronide (E/sub 3/G) and pregnanediol-3-glucuronide (P/sub 2/G) in first morning urine (FMU) for establishing a prognosis of the early pregnancy outcome was evaluated in 106 patients that became pregnant. Microaliquots of FMU were serially assayed from day 3 of the conception cycle until day 80 of pregnancy. The E/sub 3/G and P/sub 2/G profiles of 19 pregnancies which terminated in spontaneous abortion with either a diagnosis of the blighted ovum syndrome (n = 11) or presumption of a corpus luteum/trophoblast failure (n = 8) have been compared with those of clinically normal pregnancies (n = 87). Normal pregnancies displayed typical patterns of E/sub 3/G and P/sub 2/G development, while variations were observed in abortive events that reflected changes of the fetoplacental unit.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Centro de Educacion Medica e Investigaciones Clinicas, Buenos Aires, Argentina
OSTI Identifier:
5943309
Resource Type:
Journal Article
Resource Relation:
Journal Name: Fert. Steril.; (United States); Journal Volume: 42:5
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; ESTRIOL; RADIOIMMUNOASSAY; PREGNANCY; DIAGNOSIS; PREGNANEDIOL; REPRODUCTIVE DISORDERS; ABORTION; PATIENTS; ESTRANES; ESTROGENS; HORMONES; HYDROXY COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; ORGANIC COMPOUNDS; PREGNANES; RADIOASSAY; RADIOIMMUNOLOGY; STEROID HORMONES; STEROIDS; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550901 - Pathology- Tracer Techniques

Citation Formats

Mendizabal, A.F., Quiroga, S., Farinati, Z., Lahoz, M., and Nagle, C. Hormonal monitoring of early pregnancy by a direct radioimmunoassay of steroid glucuronides in first morning urine. United States: N. p., 1984. Web.
Mendizabal, A.F., Quiroga, S., Farinati, Z., Lahoz, M., & Nagle, C. Hormonal monitoring of early pregnancy by a direct radioimmunoassay of steroid glucuronides in first morning urine. United States.
Mendizabal, A.F., Quiroga, S., Farinati, Z., Lahoz, M., and Nagle, C. Thu . "Hormonal monitoring of early pregnancy by a direct radioimmunoassay of steroid glucuronides in first morning urine". United States. doi:.
@article{osti_5943309,
title = {Hormonal monitoring of early pregnancy by a direct radioimmunoassay of steroid glucuronides in first morning urine},
author = {Mendizabal, A.F. and Quiroga, S. and Farinati, Z. and Lahoz, M. and Nagle, C.},
abstractNote = {The usefulness of the direct 4-hour radioimmunoassay of estriol-16-glucuronide (E/sub 3/G) and pregnanediol-3-glucuronide (P/sub 2/G) in first morning urine (FMU) for establishing a prognosis of the early pregnancy outcome was evaluated in 106 patients that became pregnant. Microaliquots of FMU were serially assayed from day 3 of the conception cycle until day 80 of pregnancy. The E/sub 3/G and P/sub 2/G profiles of 19 pregnancies which terminated in spontaneous abortion with either a diagnosis of the blighted ovum syndrome (n = 11) or presumption of a corpus luteum/trophoblast failure (n = 8) have been compared with those of clinically normal pregnancies (n = 87). Normal pregnancies displayed typical patterns of E/sub 3/G and P/sub 2/G development, while variations were observed in abortive events that reflected changes of the fetoplacental unit.},
doi = {},
journal = {Fert. Steril.; (United States)},
number = ,
volume = 42:5,
place = {United States},
year = {Thu Nov 01 00:00:00 EST 1984},
month = {Thu Nov 01 00:00:00 EST 1984}
}
  • Antibodies to estriol-16..cap alpha..-(..beta..-D-glucuronide) were raised in sheep with the use of keyhole limpet hemocyanin and bovine serum albumin conjugates of estriol-16..cap alpha..-(..beta..-D-glucuronide). A simple, rapid method is presented for direct radioimmunoassay of estriol-16..cap alpha..-(..beta..-D-glucuronide) in urine with dextran-coated charcoal used for separation of free from bound and deionized water used for dilutions. The method is thrifty in its use of reagents. The assay has been evaluated in the pregnancy range, and the sensitivity has been extended into the range necessary for monitoring induction of ovulation with pergonal.
  • A direct radioimmunoassay which does not require hydrolysis or chromatography has been developed for estriol-16..cap alpha..-glucosiduronate in pregnancy plasma and urine. The antigen used in the development of this antiserum was prepared by joining the carboxylic acid group of estriol-16..cap alpha..-glucosiduronate covalently to the epsilon-amino group of the lysine residues in bovine serum albumin. Results indicate that the direct radioimmunoassay yields levels of estriol-16..cap alpha..-glucosiduronate in late pregnancy urine that are comparable to those obtained by more elaborate procedures involving chromatographic separation of the estriol conjugates, followed by enzymic hydrolysis and measurement of the freed estriol. In addition, good correlationmore » was found between the total estrogen values in urine obtained by currently used chemical procedures and urinary levels of estriol-16..cap alpha..-glucosiduronate. The renal clearance of estriol-16..cap alpha..-glucosiduronate was determined in eight normal women during the third trimester, and the mean value +-S.D. was 404 +- 81 ml. per minute (range, 248 to 494). This method is suitable for the evaluation of variations in the plasma and urine levels of estriol-16..cap alpha..-glucosiduronate in pregnancy and offers significant advantages over the presently used chemical methods for monitoring fetal well-being.« less
  • Assays measuring immunoreactive estrone glucuronide (E/sub 1/G), estradiol-3-glucuronide (E/sub 2/-3G), estradiol-17..beta..-glucuronide (E/sub 2/-17G), estriol-3-glucuronide (E/sub 3/-3G), estriol-16..cap alpha..-glucuronide (E/sub 3/-16G), and pregnanediol-3..cap alpha..-glucuronide (Pd-3G) directly in diluted urine were developed and validated. These estrogen and pregnanediol glucuronide fractions were measured in aliquots of 24-hour and overnight samples of urine collected daily from seven women for one menstrual cycle. Urinary hormone excretion was correlated with daily serum estradiol (E/sub 2/), progesterone (P), and lutenizing hormonee (LH) levels. A sharp midcycle LH peak preceded by a preovulatory rise in serum E/sub 2/ and followed by luteal phase serum P levels were notedmore » in each of the seven apparently ovulatory cycles. Twenty-four-hour and overnight urinary excretion patterns of estrogen glucuronides were similar to those of serum E/sub 2/. Of the five estrogen glucuronide fractions tested, excretion of E/sub 2/-17G exhibited the earliest and steepest ascending slope of the preovulatory estrogen surge and correlated best with serum E/sub 2/ levels. Urinary excretion of E/sub 1/-G, E/sub 2/-3G, and E/sub 3/-16G also showed an early and steep preovulatory rise and preceded that of E/sub 3/-3G, whereas urinary excretion of E/sub 3/-3G exhibited the poorest correlation with serum E/sub 2/ concentrations. The urinary excretion of Pd-3G rose parallel to serum P levels and was markedly elevated 2 to 3 days after the midcycle LH peak in both 24-hour and overnight collections of urine. These results indicate that among the urinary estrogen conjugate fractions tested, E/sub 2/-17G is the one that most suitably predicts ovulation.« less
  • Steroid metabolism in the skin of mature male African catfish, Clarias gariepinus, reared in the laboratory, was studied in vitro by tissue incubations with (/sup 3/H)pregnenolone, (/sup 3/H)dehydroepiandrosterone, (/sup 3/H)17 alpha-hydroxyprogesterone, (/sup 3/H)androstenedione, (/sup 14/C)11 beta-hydroxyandrostenedione, and (/sup 3/H)testosterone as precursors. While pregnenolone was not converted to any other steroid, dehydroepiandrosterone was transformed mainly to 5-androstene-3 beta, 17 beta-diol. The products of 17 alpha-hydroxyprogesterone incubations were 5 beta-pregnane-3 alpha,17 alpha-diol-20-one, 5 beta-pregnane-3 alpha,17 alpha, 20 beta-triol, and 5 beta-pregnan-17 alpha-o1-3,20-dione. The major steroids of androstenedione incubations were etiocholanolone, testosterone, and androsterone. Testosterone was converted mainly to etiocholanolone and androstenedione, andmore » only small quantities of 11 beta-hydroxytestosterone, 11-ketotestosterone, and 11-ketoandrostenedione were the metabolites found in 11 beta-hydroxyandrostenedione incubation. These results demonstrated the presence of the enzymes 5 alpha- and 5 beta-reductases and 3 alpha-, 11 beta-, 17 beta-, and 20 beta-hydroxysteroid dehydrogenases in the skin. From enzymehistochemical results it appeared that the steroid conversions take place in the epithelial cells. Moreover, the presence of UDP-glucose dehydrogenase, an enzyme involved in the synthesis of glucuronic acid, in these cells indicates the possibility of steroid glucuronide formation. Indeed significant amounts of water-soluble steroid conjugates, particularly 5 beta-dihydrotestosterone- and testosterone-glucuronide, were found in the incubations with androstenedione and testosterone, indicating the presence of the UDP-glucuronosyl transferase in the catfish skin.« less
  • Paired blood and urine samples were obtained from patients between the sixth and 14th weeks of normal pregnancy. The levels of intact human chorionic gonadotropin (hCG), and of the free alpha and beta subunits, were measured by specific radioimmunoassays. There was a close association between blood and urine levels of intact hCG and of the alpha subunit of hCG, but no relation between the levels of beta subunit in these sites. These findings suggest that the use of beta subunit assays may give discrepant results according to the fluid examined. By contrast, measurement of intact hCG appears to give similarmore » results in blood and urine.« less