B-lymphocyte differentiation in lethally irradiated and reconstituted mice. II. Recovery of humoral immune responsiveness. [X radiation]

Journal Article · Tue Mar 01 04:00:00 EST 1977 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(77)90273-8· OSTI ID:7310806

Rozing, J; Brons, N H.C.; Benner, R

The recovery of humoral immune responsiveness was studied in lethally irradiated, fetal liver-reconstituted mice. By means of both membrane fluorescence and antibody formation to sheep red blood cells (SRBC) as a functional assay, the rate of recovery of the compartments of B and T lymphocytes was determined in various lymphoid organs. The recovery of the immunoglobulin-positive (B) cell compartment after irradiation and reconstitution started in the spleen. This organ was also found to be the first in which the recovery of the B-cell population was completed. The interval between the recovery of the B-cell population in the spleen and that in the other organs tested was found to increase when the irradiated mice were reconstituted with spleen colony cells instead of fetal liver cells. This proved to be caused by the number and nature of the reconstituting hemopoietic stem cells. The immunoglobulin-positive (B) cells were found to appear before SRBC-reactive B cells could be demonstrated in spleen, lymph nodes, and Peyer's patches. The appearance of T lymphocytes in the various lymphoid organs required even more time. By means of cell transfer experiments, a sequential appearance of the precursors of anti-SRBC IgM-, IgG-, and IgA-plaque-forming cells could be demonstrated in spleen, bone marrow, lymph nodes, and Peyer's patches.

• Research Organization: Univ., Rotterdam
• OSTI ID: 7310806
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 29:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
GLOBULINS
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IMMUNOGLOBULINS
IONIZING RADIATIONS
IRRADIATION
LETHAL IRRADIATION
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MICE
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RECOVERY
RODENTS
SOMATIC CELLS
SPLEEN
STEM CELLS
TISSUES
VERTEBRATES
X RADIATION