VH repertoire in progeny of long term lymphoid-cultured cells used to reconstitute immunodeficient mice

Journal Article · Mon May 01 04:00:00 EDT 1989 · J. Immunol.; (United States)

OSTI ID:6073165

Denis, K A; Timson, L K; Witte, O N

VH gene utilization in the progeny of long term lymphoid-cultured cells used for reconstitution of severe combined immunodeficient mice under varying conditions was determined. Hybridomas made from the spleens of these animals were evaluated for clonality and donor origin and a panel of 146 independent hybridomas were subsequently examined for VH expression. Hybridomas derived from the spleens of SCID mice reconstituted with fresh cells, used as a control, utilized VH families in proportion to their numerical representation in the genome. However, hybridomas from the spleens of mice reconstituted with long term cultured cells utilized a predominance of the two VH gene families most proximal to JH, characteristic of cells early in B lymphocyte development. Coinjection of thymocytes with cultured fetal liver cells, to provide good levels of T lymphocytes, did not alter this pattern of VH utilization. Irradiation (3 Gy) of the mice before cultured cell injection, which leads to more complete reconstitution of the B cell compartment, was effective in removing this bias in the VH repertoire. Hybridomas derived from these mice expressed their VH genes more in proportion to family size, characteristic of cells later in B lymphocyte development. In this manner, long term lymphoid-cultured cells can be used to study the transitions that occur in VH repertoire expression which appear to be mediated by either B lymphocyte developmental microenvironment or population size.

• Research Organization: Howard Hughes Medical Institute, Los Angeles, CA (USA)
• OSTI ID: 6073165
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 142:9; ISSN JOIMA
• Country of Publication: United States
• Language: English

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560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CELL PROLIFERATION
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GENE REGULATION
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GLOBULINS
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IMMUNOGLOBULINS
LEUKOCYTES
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MOSAICISM
ORGANIC COMPOUNDS
PROGENY
PROTEINS
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
THYMOCYTES
VERTEBRATES