A repertoire of monoclonal antibodies with human heavy chains from transgenic mice

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.17.6709· OSTI ID:7195050

Brueggemann, M; Teale, C; Surani, M A ^[1]; Caskey, H M; Williams, G T; Neuberger, M S ^[2]; Waldmann, H ^[3]

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1. Institute of Animal Physiology and Genetics Research, Cambridge (England)
2. Medical Research Council Laboratory of Molecular Biology, Cambridge (England)
3. Univ. of Cambridge (England)

The introduction of human immunoglobulin gene segments in their unrearranged configuration into the germ line of mice might allow the production of a repertoire of human antibodies. Such transgenic mice could be used for the production of human monoclonal antibodies against human antigens. To test the feasibility of this approach, mice were created that carry a human heavy-chain minilocus comprising unrearranged immunoglobulin variable, diversity, and joining elements linked to a human {mu}-chain gene. The gene segments of this minilocus are rearranged in a large proportion of cells in thymus and spleen but not in nonlymphoid tissue. Some 4% of the B lymphocytes synthesize human {mu} chains resulting in a serum titer of about 50 {mu}g of transgenic IgM antibody per ml. Hybridomas were established from the transgenic mice that stably secreted several micrograms of antibodies containing human {mu} heavy chains per milliliter.

• OSTI ID: 7195050
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:17; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
CELL DIFFERENTIATION
CHIMERAS
DNA HYBRIDIZATION
ENZYME IMMUNOASSAY
IMMUNOGLOBULINS
MAN
MICE
PHOSPHORUS 32
SPLEEN
THYMUS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
GLOBULINS
HYBRIDIZATION
IMMUNOASSAY
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MOSAICISM
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques