A repertoire of monoclonal antibodies with human heavy chains from transgenic mice
- Institute of Animal Physiology and Genetics Research, Cambridge (England)
- Medical Research Council Laboratory of Molecular Biology, Cambridge (England)
- Univ. of Cambridge (England)
The introduction of human immunoglobulin gene segments in their unrearranged configuration into the germ line of mice might allow the production of a repertoire of human antibodies. Such transgenic mice could be used for the production of human monoclonal antibodies against human antigens. To test the feasibility of this approach, mice were created that carry a human heavy-chain minilocus comprising unrearranged immunoglobulin variable, diversity, and joining elements linked to a human {mu}-chain gene. The gene segments of this minilocus are rearranged in a large proportion of cells in thymus and spleen but not in nonlymphoid tissue. Some 4% of the B lymphocytes synthesize human {mu} chains resulting in a serum titer of about 50 {mu}g of transgenic IgM antibody per ml. Hybridomas were established from the transgenic mice that stably secreted several micrograms of antibodies containing human {mu} heavy chains per milliliter.
- OSTI ID:
- 7195050
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:17; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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550201* - Biochemistry- Tracer Techniques