B lymphocyte differentiation in lethally irradiated and reconstituted mice. I. The effect of strontium-89 induced bone marrow aplasia on the recovery of the B cell compartment in the spleen

Journal Article · Tue Jun 01 04:00:00 EDT 1976 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(76)90133-7· OSTI ID:7333949

Rozing, J; Buurman, W A; Benner, R

The influence of /sup 89/Sr-treatment on the recovery of the B cell compartment in lethally irradiated, fetal liver reconstituted mice was studied by means of membrane fluorescence, /sup 89/Sr is a bone-seeking radio-isotope which causes in a dose of 3 ..mu..Ci /sup 89/Sr/g body weight a depletion of all nucleated cells, including immunoglobulin-bearing (B) cells, of the bone marrow. Treatment of irradiated and fetal liver reconstituted mice with 3 ..mu..Ci /sup 89/Sr/g body weight immediately and at 17 days after irradiation and reconstitution prevented recovery of the nucleated cell population, including B cells, in the bone marrow. In the spleen of such mice both nucleated cells and B cells reappeared at day 7 and 14 respectively. The B cell population in the spleen did not recover up to normal values during the experimental period of 45 days. It is concluded that B cell differentiation in lethally irradiated, fetal liver reconstituted mice can take place outside the bone marrow. The efficiency of this extra-medullary differentiation is discussed. The conclusion was drawn that mice with a /sup 89/Sr-induced bone marrow aplasia are able to generate B lymphocytes. Consequently the bone marrow microenvironment seems not to be obligate to the differentiation of B lymphocytes. The peripheral lymphoid organs of such mice were found to be unable to compensate completely for the absence of B lymphocyte production in the bone marrow.

• Research Organization: Erasmus Univ., Rotterdam
• OSTI ID: 7333949
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 24:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560162* -- Radionuclide Effects
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
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BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
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BODY FLUIDS
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CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
GLANDS
HEMATOPOIETIC SYSTEM
INTERMEDIATE MASS NUCLEI
IRRADIATION
ISOTOPES
LETHAL IRRADIATION
LEUKOCYTES
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MAMMALS
MICE
NUCLEI
ORGANS
RADIATION EFFECTS
RADIOISOTOPES
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SPLEEN
STRONTIUM 89
STRONTIUM ISOTOPES
TISSUES
VERTEBRATES