Long-term effects of in vitro sensitization on immune reactivity of lymphocytes: generation of suppressor and helper T cells. [X radiation, mice]

Journal Article · Fri Apr 01 04:00:00 EST 1977 · J. Immunol.; (United States)

OSTI ID:7310801

Chan, E L; Kan, P B; Mishell, R I

Mouse spleen cells were cultured for 5 days with or without HRBC. Cultured cells were ''parked'' in irradiated syngeneic recipients for 3 weeks and then tested for their immunologic reactivity in vitro. We found that spleen cells from recipients of HRBC-sensitized cells (S) as well as spleen cells from recipients of control unsensitized cells (U) possessed radiosensitive suppressor and radioresistant helper activities. Suppressor activity was observed by the capacity of unirradiated S and U spleen cells to inhibit the in vitro generation of IgM and IgG PFC by spleen cells primed in vivo to HRBC or to LacKLH. Helper activity was shown by the capacity of the irradiated S and U cells to restore IgM and IgG PFC responses of in vivo primed, T-depleted spleen cells to HRBC, LacHRBC, and LacCRBC. Both suppressor and helper activities were mediated by T cells. The possibilities that immunologically specific or nonspecific mechanisms account for these phenomena are discussed.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 7310801
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 118:4; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IMMUNE REACTIONS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MICE
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
THYMOCYTES
VERTEBRATES
X RADIATION