Radiosensitivity of the helper cell function. [X radiation, mice]

Journal Article · Wed Nov 01 04:00:00 EST 1978 · J. Immunol.; (United States)

OSTI ID:6592122

Agarossi, G; Pozzi, L; Mancini, C; Doria, G

The helper function of T cells primed and irradiated in vivo was tested in vitro by the Mishell-Dutton technique. Spleen cells from mice carrier-primed with HRBC and exposed to 50 to 2000 rads of x-radiation were assayed for their ability to help syngeneic normal spleen cells to mount an in vitro anti-hapten antibody response after stimulation with the conjugate TNP-HRBC. The anti-TNP response was evaluated by the Jerne technique. The helper activity was titrated by adding graded numbers of carrier-primed spleen cells to a constant number of normal spleen cells. The slope of the initial linear portion of the response-cell dose titration curve was taken as an estimate of the helper activity and found to decrease with increasing the x-ray dose. The curve describing the remaining helper activity as a function of the radiation dose shows the presence of two components, one radiosensitive, the other, radioresistant. This suggests the existence either of helper cells at different stages of activation or of two cell subpopulations participating in the helper function.

• Research Organization: CNEN-Euratom Immunogenetics Group, Rome
• OSTI ID: 6592122
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 121:5; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ERYTHROCYTES
IMMUNE REACTIONS
IN VITRO
IN VIVO
IONIZING RADIATIONS
MAMMALS
MICE
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RODENTS
SOMATIC CELLS
SPLEEN CELLS
THYMOCYTES
VERTEBRATES
X RADIATION