Generation of specific helper cells and suppressor cells in vitro for the IgE and IgG antibody responses
Normal splenic lymphocytes from BDF/sub 1/ mice were cultured on ovalbumin (OA)-bearing syngeneic peritoneal adherent cells for 5 days and their subsequent helper function was tested by an adoptive transfer technique. Lymphocytes harvested from the culture were mixed with DNP-KLH-primed spleen cells and transferred into irradiated syngeneic mice followed by challenge with DNP-OA. The results showed that the cultured lymphocytes has helper function for both IgE and IgG anti-DNP antibody responses. Depletion of mast cells and T cells in the peritoneal adherent cell preparations did not affect the generation of helper cells in the culture. The helper function of the cultured lymphocytes was abolished by the treatment with anti-theta antiserum and complement and was specific for ovalbumin.
- Research Organization:
- Johns Hopkins Univ., Baltimore
- OSTI ID:
- 7349003
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 117:1; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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