Erythrocyte 2,3-DPG following cobalt intoxication
An intracellular factor which alters the P/sub 50/ (the partial pressure of oxygen at which one-half of the hemoglobin concentration is saturated with oxygen) is 2,3-diphosphoglycerate (DPG). The net effect of increased amounts of DPG is an increase in the P/sub 50/ and a decrease in the affinity of hemoglobin for oxygen. Thus it has been suggested that the delivery of oxygen to tissue may be enhanced by the presence of increased amounts of DPG in the erythrocyte during mild stages of hypoxia. Conversely, decreased amounts of DPG might result in an impaired release of oxygen to tissues. Cobalt ion has a stimulatory effect on erythropoiesis in mammals. Thus, it was of interest to study DPG at a time prior to detection of a rise in serum erythropoietin. Erythrocyte DPG levels in mice were measured prior to and six hours following injection of cobalt chloride or saline. Hemoglobin concentration, DPG, and erythrocyte stimulating factor were measured as well as the amount of Fe 59 incorporated into newly formed red cells. Serum from female mice given 5 ..mu..m of cobalt chloride was found to contain a nondialyzable factor that stimulated erythropoiesis to a degree comparable to 0.8 units ESF/ml serum. Serum from female mice given saline was not effective in promoting an increased rate of erythropoiesis. DPG concentrations were found to be similar in both the cobalt- and saline-treated mice. The results suggest that the stimulatory effect of cobalt on erythropoiesis does not involve an inhibition of red cell glycolytic respiration. Also, in mice treated with erythropoietic dosages of cobalt, the capacity of the circulating erythrocytes for oxygen transport does not appear to be altered at a time prior to the detection of elevated serum ESF levels.
- OSTI ID:
- 7308364
- Journal Information:
- J. Elisha Mitchell Sci. Soc.; (United States), Journal Name: J. Elisha Mitchell Sci. Soc.; (United States) Vol. 92:2; ISSN JEMSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551000 -- Physiological Systems
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
COBALT
COBALT CHLORIDES
COBALT COMPOUNDS
CRYOGENIC FLUIDS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELEMENTS
ERYTHROCYTES
ERYTHROPOIESIS
ERYTHROPOIETIN
EVEN-ODD NUCLEI
FLUIDS
GLOBIN
GLYCOLYSIS
HALIDES
HALOGEN COMPOUNDS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INHIBITION
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
ISOTOPES
MAMMALS
METALS
MICE
NONMETALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN
PIGMENTS
PORPHYRINS
PROTEINS
QUANTITY RATIO
RADIOISOTOPES
RESPIRATION
RODENTS
SATURATION
STIMULATION
TRANSITION ELEMENT COMPOUNDS
TRANSITION ELEMENTS
VERTEBRATES