Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae

Journal Article · Sun May 01 04:00:00 EDT 1977 · Genetics; (United States)

OSTI ID:7306769

Prakash, L; Prakash, S

We have isolated mutants sensitive to methyl methanesulfonate (MMS) in Saccharomyces cerevisiae. Alleles of rad1, rad4, rad6, rad52, rad55 and rad57 were found among these mms mutants. Twenty-nine of the mms mutants which complement the existing radiation-sensitive (rad and rev) mutants belong to 22 new complementation groups. Mutants from five complementation groups are sensitive only to MMS. Mutants of 11 complementation groups are sensitive to uv or x rays in addition to MMS, mutants of six complementation groups are sensitive to all three agents. The cross-sensitivities of these mms mutants to uv and x rays are discussed in terms of their possible involvement in DNA repair. Sporulation is reduced or absent in homozygous diploids of mms mutants from nine complementation groups.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7306769
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 86:1; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOMASS
DNA
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
ESTERS
FUNGI
IONIZING RADIATIONS
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
RENEWABLE ENERGY SOURCES
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SENSITIVITY
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION
X RADIATION
YEASTS