Characterization of an MMS sensitive mutant of Saccharomyces cerevisiae
We have characterized a methyl methanesulfonate sensitive mutant of the yeast Saccharomyces cerevisiae in order to learn more about DNA repair and mutagenesis in this organism. The mutation, designated mms3-1, also confers sensitivity to ultraviolet light and to ethyl methanesulfonate in both haploids and homozygous diploids. Its effect on ..gamma..-ray sensitivity, however, is a function of the ploidy of the cell and its effect on induced mutation is a function of both the ploidy of the cell and the nature of the inducing agent. Our major findings are discussed. Our data indicate that: (1) Saccharomyces cerevisiae has an error prone pathway for the repair of uv damage controlled by the MMS3 gene product operating in and only in, and possibly induced by conditions present only in, a/..cap alpha.. diploids; (2) in diploids, at least, there exists at least one step in the error prone repair of uv induced damage which is different from a step in the error prone repair of EMS induced damage; (3) a/..cap alpha.. mms3-1/mms3-1 diploids may be defective in a step common to the repair of mutagenic lesions following uv irradiation and lethal lesions following ..gamma.. irradiation; and (4) there are steps in the repair of MMS induced lethal damage that are different from steps in the repair of EMS induced lethal damage.
- Research Organization:
- Rochester Univ., NY (USA). Dept. of Radiation Biology and Biophysics
- DOE Contract Number:
- EY-76-C-02-3490
- OSTI ID:
- 5676642
- Report Number(s):
- UR-3490/LCP-19
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DATA
DATA FORMS
DNA
ELECTROMAGNETIC RADIATION
EMS
ESTERS
EXPERIMENTAL DATA
FUNGI
GAMMA RADIATION
GENETIC CONTROL
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTANTS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEST CONTROL
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION
YEASTS