A/. cap alpha. -specific effect of the mms3 mutation on ultraviolet mutagenesis in Saccharomyces cerevisiae
A new gene involved in error-prone repair of ultraviolet (uv) damage has been identified in Saccharomyces cerevisiae by the mms3-1 mutation. Uv-induced reversion is reduced in diploids that are homozygous for mms3-1, only if they are also heterozygous (MATa/MAT..cap alpha..) at the mating type locus. The mms3-1 mutation has no effect on uv-induced reversion either in haploids or MATa/MAT..cap alpha.. or MAT..cap alpha../MAT..cap alpha.. diploids. The mutation confers sensitivity to uv and methyl methane sulfonate in both haploids and diploids. Even though mutation induction by uv is restored to wild-type levels in MATa/MATa mms3-1/mms3-1 or MAT..cap alpha../MAT..cap alpha.. mms3-1/mms3-1 diploids, such strains still retain sensitivity to the lethal effects of uv. Survival after uv irradiation in mms3-1 rad double mutant combinations indicates that mms3-1 is epistatic to rad6-1 whereas non-epistatic interactions are observed with rad3 and rad52 mutants. When present in the homozygous state in MATa/MAT..cap alpha.. his1-1/his1-315 heteroallelic diploids, mms3-1 was found to lower uv-induced mitotic recombination.
- Research Organization:
- Univ. of Rochester, NY
- DOE Contract Number:
- AC02-76EV03490
- OSTI ID:
- 6449370
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 146:2; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
550700 -- Microbiology
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
ELECTROMAGNETIC RADIATION
FUNGI
GENE MUTATIONS
GENES
GENETICS
MICROORGANISMS
MUTANTS
MUTATIONS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
REVERTANTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
YEASTS