Effects of the rad52 gene on recombination in Saccharomyces cerevisiae
Effects of the rad 52 mutation in Saccharomyces cerevisiae on meiotic, ..gamma..-ray-induced, uv-induced and spontaneous mitotic recombination were studied. The rad52/rad52 diploids undergo premeiotic DNA synthesis; sporulation occurs but inviable spores are produced. Both intra and intergenic recombination during meiosis were examined in cells transferred from sporulation medium to vegetative medium at different time intervals. No intragenic recombination was observed at the his1-1/his1-315 and trp-5-2/trp5-48 heteroalleles. Gene-centromere recombination also was not observed in rad/52/rad52 diploids. No ..gamma..-ray- or uv-induced intragenic mitotic recombination is seen in rad52/rad52 diploids. The rate of spontaneous mitotic recombination is lowered five-fold at the his1-1/his1-315 and leu1-c/leu1-12 heteroalleles. Spontaneous reversion rates of both his1-1 and his1-315 were elevated 10 to 20 fold in rad52/rad52 diploids. The RAD52 gene function is required for spontaneous mitotic recombination, uv- and ..gamma..-ray-induced mitotic recombination and mitotic recombination.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 5369372
- Journal Information:
- Genetics; (United States), Journal Name: Genetics; (United States) Vol. 94:1; ISSN GENTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550700 -- Microbiology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DNA
ELECTROMAGNETIC RADIATION
FUNGI
GENE MUTATIONS
GENE RECOMBINATION
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SPORES
ULTRAVIOLET RADIATION
YEASTS