Effects of the rad52 gene on recombination in Saccharomyces cerevisiae. [Comparison of. gamma. -, uv-induced meiotic and spontaneous mitotic recombination]
Effects of the rad52 mutation in Saccharomyces cerevisiae on meiotic, ..gamma..-ray-induced, uv-induced, and spontaneous mitotic recombination were studied. The rad52/rad52 diploids undergo premeiotic DNA synthesis; sporulation occurs but inviable spores are produced. Intra- and intergenic recombination during meiosis were examined in cells transferred from sporulation medium to vegetative medium at different time intervals. No intragenic recombination was observed at the hisl-1/hisl-315 and trp5-2/trp5-48 heteroalleles. Gene-centromere recombination was also not observed in rad52/rad52 diploids. No ..gamma..-ray-induced intragenic mitotic recombination is seen in rad52/rad52 diploids and uv-induced intragenic recombination is greatly reduced. However, spontaneous mitotic recombination is not similarly affected. The RAD52 gene thus functions in recombination in meiosis and in ..gamma..-ray and uv-induced mitotic recombination but not in spontaneous mitotic recombination.
- Research Organization:
- Rochester Univ., NY (USA). Dept. of Biology; Rochester Univ., NY (USA). Dept. of Radiation Biology and Biophysics
- DOE Contract Number:
- EY-76-C-02-3490
- OSTI ID:
- 6162720
- Report Number(s):
- UR-3490-1571
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
550700 -- Microbiology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL DIVISION
COMPARATIVE EVALUATIONS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FUNGI
GAMMA RADIATION
GENE MUTATIONS
GENE RECOMBINATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENOTYPE
GRAPHS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
MEIOSIS
MICROORGANISMS
MITOSIS
MUTATIONS
NUMERICAL DATA
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SPONTANEOUS MUTATIONS
ULTRAVIOLET RADIATION
YEASTS
550700 -- Microbiology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL DIVISION
COMPARATIVE EVALUATIONS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FUNGI
GAMMA RADIATION
GENE MUTATIONS
GENE RECOMBINATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENOTYPE
GRAPHS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
MEIOSIS
MICROORGANISMS
MITOSIS
MUTATIONS
NUMERICAL DATA
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SPONTANEOUS MUTATIONS
ULTRAVIOLET RADIATION
YEASTS