Two DNA repair and recombination genes in Saccharomyces cerevisiae, RAD52 and RAD54, are induced during meiosis

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.9.7.3101· OSTI ID:5623786

Cole, G M; Mortimer, R K ^[1]; Schild, D ^[2]

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1. Univ. of California, Berkeley (United States)
2. Lawrence Berkeley Lab., CA (United States)

The DNA repair and recombination genes of Saccharomyces cerevisiae, RAD52 and RAD54, were transcriptionally induced approximately 10- to 15-fold in sporulating MATa/{alpha} cells. Congenic MATa/a cells, which did not sporulate, did not show similar increases. Assays of {beta}-galactosidase activity in strains harboring either a RAD52- or RAD54-lacZ gene fusion indicated that this induction occurred at a time concomitant with a commitment to meiotic recombination, as measured by prototroph formation from his1 heteroalleles.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5623786
• Journal Information: Molecular and Cellular Biology; (United States), Vol. 9:7; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA REPAIR
MOLECULAR BIOLOGY
GENE RECOMBINATION
BACTERIAL SPORES
BIOLOGICAL FUNCTIONS
GENES
MEIOSIS
SACCHAROMYCES CEREVISIAE
TRANSCRIPTION
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL DIVISION
EUMYCOTA
FUNGI
MICROORGANISMS
PLANTS
RECOVERY
REPAIR
SACCHAROMYCES
SPORES
YEASTS
550200* - Biochemistry
551000 - Physiological Systems