Mitotic chromosome loss in a radiation-sensitive strain of the yeast Saccharomyces cerevisiae

Journal Article · Tue Sep 01 00:00:00 EDT 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.78.9.5778· OSTI ID:5821023

Mortimer, R K; Contopoulou, R; Schild, D

Cells of Saccharomyces cerevisiae with mutations in the RAD52 gene have previously been shown to be defective in meiotic and mitotic recombination, in sporulation, and in repair of radiation-induced damage to DNA. In this study we show that diploid cells homozygous for rad52 lose chromosomes at high frequencies and that these frequencies of loss can be increased dramatically by exposure of these cells to x-rays. Genetic analyses of survivors of x-ray treatment demonstrate that chromosome loss events result in the conversion of diploid cells to cells with near haploid chromosome numbers.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 5821023
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:9; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560113 -- Radiation Effects on Biochemicals-- In Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
CELL DIVISION
CHROMOSOME LOSSES
CHROMOSOMES
DNA
ELECTROMAGNETIC RADIATION
FUNGI
GENETICS
IONIZING RADIATIONS
LOSSES
MEIOSIS
MICROORGANISMS
MITOSIS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
SACCHAROMYCES
X RADIATION
YEASTS