Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and. cap alpha. factor pheromones

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.1.11· OSTI ID:5725054

Chan, R K; Otte, C A

Eight independently isolated mutants which are supersensitive (Sst/sup -/) to the G1 arrest induced by the tridecapeptide pheromone ..cap alpha.. factor were identified by screening mutagenized Saccharomyces cerevisiae MATa cells on solid medium for increased growth inhibition by ..cap alpha.. factor. These mutants carries lesions in two complementation groups, sst1 and sst2. Mutations at the sst1 locus were mating type specific: MATa sst1 cells were supersensitive to ..cap alpha.. factor, but MAT..cap alpha.. sst1 cells were not supersensitive to a factor. In contrast, mutations at the sst2 locus conferred supersensitivity to the pheromones of the opposite mating type on both MATa and MAT..cap alpha.. cells. Even in the absence of added ..cap alpha.. pheromone, about 10% of the cells in exponentially growing cultures of MATa strains carrying any of three different alleles of sst2 (including the ochre mutation sst2-4) had the aberrant morphology (''shmoo'' shape) that normally develops only after MATa cells are exposed to ..cap alpha.. factor. This ''self-shmooing'' phenotype was genetically linked to the sst2 mutations, although the leakiest allele isolated (sst2-3) did not display this characteristic. Normal MATa/MAT..cap alpha.. diploids do not respond to pheromones; diploids homozygous for an sst2 mutation (MATa/MAT..cap alpha.. sst2-1/sst2-1) were still insensitive to ..cap alpha.. factor. The sst1 gene was mapped to within 6.9 centimorgans of his6 on chromosome IX. The sst2 gene was unlinked to sst1, was not centromere linked, and was shown to be neither linked nor centromere distal to MAT on the right arm of chromosome III.

• Research Organization: Dept. of Microbiology, Univ. of Cincinnati College of Medicine, Cincinnati, OH 45267
• OSTI ID: 5725054
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:1; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL PATHWAYS
CELL DIVISION
CHEMICAL PREPARATION
CHROMOSOMES
CLASSIFICATION
DIPLOIDY
FUNGI
GENETIC MAPPING
MAPPING
MICROORGANISMS
MITOSIS
MOLECULAR BIOLOGY
MORPHOLOGY
MUTANTS
MUTATION FREQUENCY
PHENOTYPE
PHEROMONE
PLANT BREEDING
PLANT CELLS
PLANTS
PLOIDY
REVERTANTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SECRETION
SEPARATION PROCESSES
SYNTHESIS
YEASTS