Pleiotropic mutations at the TUP1 locus that affect the expression of mating-type-dependent functions in Saccharomyces cerevisiae

Journal Article · Tue Apr 01 04:00:00 EST 1980 · Genetics; (United States)

OSTI ID:6866548

Lemontt, J F ^[1]; Fugit, D R; MacKay, V L

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1. Oak Ridge National Lab., TN

The umr7-1 mutation, previously identified in a set of mutants that had been selected for defective uv-induced mutagenesis at CAN1, affects other cellular functions, including many of those regulated by the mating-type locus (MAT) in heterothallic Saccharomyces cerevisiae. The recessive umr7-1 allele, mapping approximately 20 cM distal to thr4 on chromosome III, causes clumpy growth in both a and ..cap alpha.. cells and has no apparent effect on a mating functions. The most striking property of a umr7 strains is their altered morphology, in which mitotic cells develop an asymmetric pear shape, like that of normal a cells induced to form shmoos by interaction with a-factor. Some a/..cap alpha..-specific diploid functions are also affected by umr7; instead of polar budding patterns, a/..cap alpha.. umr7/umr7 diploids have medial budding like a/a, ..cap alpha../..cap alpha.. and haploid strains. Moreover, a/..cap alpha.. umr7/umr7 diploids have lost the ability to sporulate and are Bar+ like a or a/a strains. Revertant studies indicate that umr7-1 is a single point mutation. The umr7 mutant fails to complement mutants of both tup1 and cyc9 and all three isolates have similar genetic and phenotypic properties. It is suggested that the product of this gene plays some common central role in the complex regulation of the expression of both MAT-dependent and MAT-independent functions.

• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6866548
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 94:4; ISSN GENTA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL VARIABILITY
ELECTROMAGNETIC RADIATION
FUNGI
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GENETIC EFFECTS
GENETIC MAPPING
GENETIC RADIATION EFFECTS
GENETIC VARIABILITY
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MICROORGANISMS
MORPHOLOGY
MUTANTS
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PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
REVERTANTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
YEASTS
ZYGOTES