Endomitotic effect of a cell cycle mutation of Saccharomyces cerevisiae

Journal Article · Sun Mar 01 04:00:00 EST 1981 · Genetics; (United States)

OSTI ID:6315617

Schild, D; Ananthaswamy, H N; Mortimer, R K

A recessive temperature-sensitive mutation of Saccharomyces cerevisiae has been isolated and shown to cause an increase in ploidy in both haploids and diploids. Genetic analysis revealed that the strain carrying the mutation was an aa diploid, although MNNG mutagenesis had been done on an a haploid strain. When the mutant strain was crossed with an ..cap alpha cap alpha.. diploid and the resultant tetraploid sporulated, some of the meiotic progeny of this tetraploid were themselves tetraploid, as shown by both genetic analysis and DNA measurements, instead of diploid as expected of tetraploid meiosis. The ability of these tetraploids to continue to produce tetraploid meiotic progeny was followed for four generations. It was found that tetraploidization was independent of sporulation temperature, but was dependent on the temperature of germination and the growth of the spores. Increase in ploidy occurred when the spores were germinated and grown at 30/sup 0/, but did not occur at 23/sup 0/. Two cycles of sporulation and growth at 23/sup 0/ resulted in haploids, which were shown to diploidize within 24 hr when grown at 30/sup 0/.

• Research Organization: Univ. of California., Berkeley
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6315617
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 97:3-4; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKYLATING AGENTS
BIOLOGY
CELL CYCLE
CELL DIVISION
FUNGI
GENETICS
MEIOSIS
MICROORGANISMS
MITOSIS
MUTATIONS
PLANTS
PLOIDY
RECESSIVE MUTATIONS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SPORES
TEMPERATURE DEPENDENCE
YEASTS