Role of ploidy in radiosensitivity of cells (experiments on yeast organisms of different species and genotypes). [Alpha particles, gamma radiation]

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Radiobiology (USSR) (Engl. Transl.); (United States)

OSTI ID:5166671

Korogodin, V I; Bliznik, K M; Kapul'tsevich, Yu G; Petin, V G; Savchenko, G V; Tolstorukov, I I

It was established that the sensitivity of artificially obtained diploid cells from natural haplonts Pichia pinus and Pichia guilliermondii is twice as sensitive to ..gamma..-rays and ..cap alpha..-particles as haploid cells and, consequently, there is no relationship to ploidy per chromosome set. The haplonts are similar to diplont Saccharomyces cerevisiae bearing the rad 51 mutation, and they are very different from S. cerevisiae of the ''wild'' type, in which haploid cells are much more radiosensitive than diploids. It was also established that the RBE of ..cap alpha..-particles for yeast of the strains studied decreases with increase in radiosensitivity, changing from 4.6 to 1.0. The following conclusions are derived: the radiation lesion to haploid and diploid yeast cells is based on similar injuries, the probability of which is determined by the size of the ''target'' (DNA content) and effectiveness of repair systems; the presence in the cell of at least two sets of chromosomes is a prerequisite for effective function of repair systems; the higher radioresistance of diploid cells, as compared to haploids, in diplont yeast is due primarily to effective function of repair systems; the greater radiosensitivity of diploid cells, as compared to haploids, in haplont yeast is apparently due to a deficiency in this yeast with regard to repair systems; repair processes play a large role in determining the RBE of radiations with different linear energy transfer.

• Research Organization: All-Union Scientific Research Inst. of Genetics and Breeding of Industrial Microorganisms, Moscow
• OSTI ID: 5166671
• Journal Information: Radiobiology (USSR) (Engl. Transl.); (United States), Journal Name: Radiobiology (USSR) (Engl. Transl.); (United States) Vol. 17:5; ISSN RADBA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALPHA PARTICLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOMASS
CHARGED PARTICLES
CHROMOSOMES
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
FUNGI
GAMMA RADIATION
IONIZING RADIATIONS
MICROORGANISMS
PLANTS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RBE
RENEWABLE ENERGY SOURCES
YEASTS