The SRS2 suppressor of rad6 mutations of Saccharomyces cerevisiae acts by channeling DNA lesions into the RAD52 DNA repair pathway

Journal Article · Sat Mar 31 23:00:00 EST 1990 · Genetics; (USA)

OSTI ID:6790649

Schiestl, R H; Prakash, S; Prakash, L ^[1]

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1. Univ. of Rochester School of Medicine, NY (USA)

rad6 mutants of Saccharomyces cerevisiae are defective in the repair of damaged DNA, DNA damage induced mutagenesis, and sporulation. In order to identify genes that can substitute for RAD6 function, the authors have isolated genomic suppressors of the UV sensitivity of rad6 deletion (rad6{Delta}) mutations and show that they also suppress the {gamma}-ray sensitivity but not the UV mutagenesis or sporulation defects of rad6. The suppressors show semidominance for suppression of UV sensitivity and dominance for suppression of {gamma}-ray sensitivity. The six suppressor mutations they isolated are all alleles of the same locus and are also allelic to a previously described suppressor of the rad6-1 nonsense mutation, SRS2. They show that suppression of rad6{Delta} is dependent on the RAD52 recombinational repair pathway since suppression is not observed in the rad6{Delta} SRS2 strain containing an additional mutation in either the RAD51, RAD52, RAD54, RAD55 or RAD57 genes. Possible mechanisms by which SRS2 may channel unrepaired DNA lesions into the RAD52 DNA repair pathway are discussed.

• OSTI ID: 6790649
• Journal Information: Genetics; (USA), Journal Name: Genetics; (USA) Vol. 124:4; ISSN 0016-6731; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
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COBALT 60
COBALT ISOTOPES
DNA
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ELECTROMAGNETIC RADIATION
EUMYCOTA
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GAMMA RADIATION
GENE MUTATIONS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MUTAGENESIS
MUTATION FREQUENCY
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PLANTS
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RADIOISOTOPES
RECOVERY
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SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
YEARS LIVING RADIOISOT
YEASTS