Inhibition of infectious Rous sarcoma virus production by a rifamycin derivative

Journal Article · Sat May 01 00:00:00 EDT 1976 · J. Virol.; (United States)

OSTI ID:7292954

Szabo, C; Bissell, M J; Calvin, M

A new rifamycin derivative, rifazone-8/sub 2/ (R-8/sub 2/), an inhibitor of viral RNA-dependent DNA polymerase, is selectively toxic to transformed chicken cells in culture. R-8/sub 2/ has now been shown to possess antiviral activity as well. The relatively nontoxic properties of R-8/sub 2/ to nontransformed cells have permitted the execution of experiments examining the effect of a rifamycin derivative on virus reproduction. Addition of low concentrations of R-8/sub 2/ (15 ..mu..g/ml) to cultures soon after Rous sarcoma virus infection prevents the spread of infection throughout the culture. This inhibition is not dependent on concomitant cellular transformation as identical results were obtained with cells infected with a transformation-defetive Rous sarcoma virus. Addition of R-8/sub 2/ to cultures in which all the cells are infected does not substantially affect the yield of physical particles as measured by RNA-dependent DNA polymerase activity and by (/sup 3/H)uridine incorporation into viral RNA. However, the infectivity of the progeny virus, as measured by focus-forming ability, is decreased 95 to 99% by R-8/sub 2/ treatment. (auth)

• Research Organization: Lawrence Berkeley Lab., CA
• OSTI ID: 7292954
• Report Number(s): LBL-4627
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 18:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANTIBIOTICS
CELL CULTURES
DISEASES
DNA
DRUGS
ENZYMES
INFECTIOUS DISEASES
INHIBITION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
POLYMERASES
REPRODUCTION
VIRUSES