Antiviral action of a rifamycin derivative: formation of Rous sarcoma virus particles deficient in 60 to 70S RNA
Journal Article
·
· J. Virol.; (United States)
OSTI ID:7208201
Rifazone-8/sub 2/ (R-8/sub 2/), a derivative of the antibiotic rifamycin-SV, is a potent inhibitor of viral reverse transcriptase and possesses a number of other interesting properties. It inhibits growth of virus-transformed cells in culture, has antiviral activity. When added to cultures in which only a fraction of the cells are infected, it prevents the spread of Rous sarcoma virus by causing production of noninfectious particles. The mechanism of antiviral activity is examined in detail. The noninfectious virions had altered buoyant density. Studies with tritiated drug showed that it was firmly bound to the virus. Most of the RNA in the noninfectious virus appeared as small species and only a minor fraction of 60 to 70S RNA was detected. The inhibition was dependent on concentration of the drug. Growth of transformed cells in rifampin had negligible effect on virus production and infectivity, suggesting that the antiviral activity of R-8/sub 2/ is in part related to the lipophilic side chain it contains. It is possible that R-8/sub 2/ associates with RSV during budding and disrupts the normal compact structure of the virion. This may prevent stabilization of the 60 to 70S RNA and lead to its degradation or the drug may act as a nucleotide polymerase inhibitor preventing synthesis of 30 to 40S subunits.
- Research Organization:
- Lawrence Berkeley Lab., CA
- OSTI ID:
- 7208201
- Report Number(s):
- LBL-5591
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 25:3; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Inhibition of infectious Rous sarcoma virus production by a rifamycin derivative
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