Caffeine enhancement of x-ray killing in cultured human and rodent cells
A 16 to 20 hr postirradiation incubation with caffeine enhances x-ray killing of rodent and human cells. Cells tested were Chinese hamster ovary (CHO-K1), lung (CHL), V79, mouse L, HeLa S3, human fibroblasts (AF288, TC171, FS9, CRL1166), and a human-hamster hybrid. The effect of caffeine on the x-ray survival curve of these cells was to remove the initial shoulder without significantly altering the mean lethal dose (D/sub 0/). This action can be achieved at caffeine concentrations which of themselves cause less than 15% killing. In randomly growing CHO-K1 cells the caffeine-sensitive process occurs with a half-time of 2 to 5 hr after irradiation. These experiments indicate the existence in human and rodent cells of caffeine-inhibited genome repair for x-ray damage.
- Research Organization:
- Univ. of Colorado Medical Center, Denver
- OSTI ID:
- 7276970
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 73:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CAFFEINE
CELL CULTURES
CELL KILLING
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HELA CELLS
HETEROCYCLIC COMPOUNDS
INHIBITION
IONIZING RADIATIONS
IRRADIATION
L CELLS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SOMATIC CELLS
SURVIVAL CURVES
X RADIATION
XANTHINES
XP CELLS