Caffeine-enhanced survival of radiation-sensitive, repair-deficient Chinese hamster cells

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576217· OSTI ID:5017863

Utsumi, H; Elkind, M M

A clone of V79 Chinese hamster cells (V79-AL162/S-10) with unique properties has been isolated after a challenge of parental cells (V79-AL162) with 1 mM ouabain. Compared with parental cells, or with other clones isolated after the ouabain challenge, these cells form smaller colonies, are more sensitive to both x rays and fission-spectrum neutrons, and respond atypically to a postirradiation treatment with caffeine. Their enhanced response to x rays results mainly from a large reduction in the shoulder of their survival curve, probably because in late S phase, the most resistant phase in the cell cycle, the survival curve of these cells has a reduced shoulder width. Caffeine, and to a lesser extent theophylline, added to the colony-forming medium immediately after exposure appreciably increases the width of the shoulder of these sensitive cells, whereas caffeine has the opposite effect on the response of normal V79 cells. Thus the unique response of the V79-AL162/S-10 cells to a radiation posttreatment with caffeine (increased survival) results from a net increase in their ability to repair damage that is otherwise lethal; caffeine treatment ordinarly prevents normal V79 cells from repairing damage that is only potentially lethal.

• Research Organization: Argonne National Lab., IL
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5017863
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 96:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANALEPTICS
ANIMAL CELLS
AROMATICS
AZAARENES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CAFFEINE
CARDIOVASCULAR AGENTS
CELL KILLING
CENTRAL NERVOUS SYSTEM AGENTS
CONNECTIVE TISSUE CELLS
DATA
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
INFORMATION
IONIZING RADIATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SOMATIC CELLS
SURVIVAL CURVES
THEOPHYLLINE
VASODILATORS
X RADIATION
XANTHINES