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Recovery after exposure to near-ultraviolet light of cells containing 5-bromodeoxyuridine. [V79 Chinese hamster cells]

Journal Article · · Biophys. J.; (United States)
;

The survival of synchronized V79 Chinese hamster cells irradiated with near-ultraviolet light after a 1-h labeling with 5-bromodeoxyuridine (BrdUrd) is highly dependent upon the cells' position in the cell cycle at the time of irradiation. In this report, we show that cells irradiated in the same S phase after BrdUrd incorporation demonstrate an ability to repair sublethal damage, in contrast to the lack of an increase in survival with dose fractionation in template-labeled cells. In addition, we show that pulse-labeled cells in S phase can repair potentially lethal damage expressed by caffeine. The kinetics of these recovery processes and the absence of a caffeine effect on the rapair of sublethal damage indicate that these two processes are to a large degree unrelated. We conclude that in template-labeled cells inadequate time to effect prereplicational repair precludes effective contributions to cell survival from other kinds of DNA repair processes.

Research Organization:
Argonne National Lab., IL
DOE Contract Number:
W-31109-ENG-38
OSTI ID:
6483539
Journal Information:
Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 34:3; ISSN BIOJA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CELL CULTURES
DNA
DRUGS
ELECTROMAGNETIC RADIATION
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IRRADIATION
MAMMALS
NEAR ULTRAVIOLET RADIATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES