Changes in repair competency after 5-bromodeoxyuridine pulse labeling and near-ultraviolet light. [V79 Chinese hamster cells]

Journal Article · Sun Jul 01 04:00:00 EDT 1979 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(79)85203-0· OSTI ID:5341396

Hagan, M P; Elkind, M M

Synchronized V79 Chinese hamster cells, pulse-labeled with 5-bromodeoxyuridine (BrdUrd), show marked changes in the sensitivity to near-ultraviolet light during the cell cycle. Cells are least sensitive during the remainder of the S-phase after the BrdUrd pulse. They become maximally sensitive in the next cell cycle when the BrdUrd-labeled DNA presumably serves as the template for replication. This is followed by a return to relative insensitivity during the remainder of that S-phase. When BrdUrd is given both near the beginning and again near the end of the same S-phase, the increase in survival does not occur until DNA synthesis progresses beyond the time when the DNA made during the second pulse serves as a template. Furthermore, cells in the resistant phases of the cell cycle are sensitized by 1-2 mM caffeine. Survival curves are shown for the various cell ages of interest and are discussed in relation to the observed changes in functional repair capacity. The data support the hypothesis that lesions in the BrdUrd-containing DNA are effectively repaired after semiconservative replication. The data indicate that saturation of repair capacity and not target multiplicity is responsible for the appearance of a shoulder on these survival curves.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5341396
• Journal Information: Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 27:1; ISSN BIOJA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CAFFEINE
CELL CULTURES
CELL CYCLE
DATA
DATA FORMS
DNA
DNA REPLICATION
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
IRRADIATION
ISOLATED VALUES
LABELLING
MAMMALS
NEAR ULTRAVIOLET RADIATION
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PULSES
PURINES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SURVIVAL CURVES
SURVIVAL TIME
SYNCHRONOUS CULTURES
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES
XANTHINES