Postreplication repair in an excision-defective mutant of Escherichia coli. Ultraviolet light-induced incorporation of bromodeoxyuridine into parental DNA

Journal Article · Wed Aug 01 04:00:00 EDT 1973 · Photochem. Photobiol., v. 18, no. 2, pp. 87-95

DOI:https://doi.org/10.1111/j.1751-1097.1973.tb06397.x· OSTI ID:4387674

Ley, R D

Ultraviolet light-induced incorporation of bromodeoxyuridine (BrdUrd) into parental DNA of an excision-defective mutant of Escherichia coli was observed by selective photolysis of bromouracil (BrUra)-containing regions in the parental DNA. It appears that the BrUra-containing regions occur only in that DNA which has served as a template for normal semiconservative replication. After an exposure at 254 nm which results in one pyrimidine dimer per 45 x 10/sup 6/ daltons, incubation in BrdUrd resulted in BrUra-containing regions ~1.5 x 10/ sup 4/ nucleotides in length at intervals of ~55 x 10/sup 6/ daltons in the parental DNA. Thus approximately one BrUra-containing region has occurred for every 1.2 pyrimidine dimers in the parental DNA. The observed incorporation of BrdUrd is interpreted in terms of a proposed model for postreplication repair in which genetic exchanges produce singlestrand gaps in the parental DNA. (auth)

• Research Organization: Argonne National Lab., IL
• NSA Number: NSA-29-005279
• OSTI ID: 4387674
• Journal Information: Photochem. Photobiol., v. 18, no. 2, pp. 87-95, Journal Name: Photochem. Photobiol., v. 18, no. 2, pp. 87-95; ISSN PHCBA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

*BUDR-- CHEMICAL RADIATION EFFECTS
*DNA-- BIOLOGICAL REPAIR
*ESCHERICHIA COLI-- DNA REPLICATION
DIMERS
MUTANTS
N48120* --Life Sciences--Radiation Effects on Biochemicals-- In Microorganisms
NUCLEOTIDES
PYRIMIDINES
STRAND BREAKS
ULTRAVIOLET RADIATION