Effects of caffeine on purine metabolism and ultraviolet light-induced lethality in cultured mammalian cells

Journal Article · Sat Dec 01 04:00:00 EST 1979 · Cancer Res.; (United States)

OSTI ID:6739958

Waldren, C A; Patterson, D

Caffeine, at doses which enhance the killing action of ultraviolet light, inhibits both de novo synthesis and the utilization of exogenous purines in cultured CHO-K1, a Chinese hamster ovary cell line. The effect is dose dependent, with a caffeine concentration of 7.5 mM producing a 90% reduction in 15 min. Interference with utilization of exogenous purines was seen as a substantial decrease in the conversion of (14C)hypoxanthine, (14C)adenine, or (14C)guanine into their respective di- and triphosphates in the presence of caffeine. Thus, one of the ways by which antimetabolites and caffeine act to enhance ultraviolet light killing may be by interference with the supply of purine nucleotides needed for repair.

• Research Organization: Univ. of Colorado Health Sciences Center, Denver
• OSTI ID: 6739958
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39:12; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENINES
AMINES
ANIMAL CELLS
BIOSYNTHESIS
CAFFEINE
CARBON 14 COMPOUNDS
CELL CULTURES
CELL KILLING
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYPOXANTHINE
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATIONS
RADIOINDUCTION
SYNERGISM
SYNTHESIS
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
XANTHINES