Nonreciprocal and host-dependent interference between two variants of influenza virus. [UV radiation]

Journal Article · Thu Apr 01 04:00:00 EST 1976 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(76)90292-0· OSTI ID:7276007

Noronha-Blob, L; Schulze, I T

Two variants of the WSN strain of influenza virus, designated F and C because they produce distinct plaque types on bovine kidney (MDBK) cells, have been found to exhibit nonreciprocal and host-dependent inteference. The interference is mediated by C particles obtained from either MDBK cells or chick embryo fibroblasts (CEF). It does not operate in CEF, and in MDBK cells the C variant is always the interference-inducer regardless of which virus enters the cell first. Standard virus prepared in CEF contains at least 20 interfering units per plaque-forming unit. Increasing the particle to infectivity ratio of the C preparation increases the number of interfering particles per plaque-forming unit. UV-inactivated C virions retain the capacity to interfere with F synthesis. Strains of virus with limited capacity to grow in MDBK cells also interfere with the synthesis of F but not of C. Preinfection of MDBK cells with F results in enhancement of early yields of C. This is not observed in CEF. Enhancement can be induced by F nonplaque formers but not by uv-inactivated virus or by other strains of influenza virus. The experiments suggest that C virions interfere with an early step in the replication of F by usurping components which are essential for virus replication and which are presented in limiting amounts in infected MDBK cells.

• Research Organization: St. Louis Univ.
• OSTI ID: 7276007
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 70:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
INACTIVATION
INFLUENZA VIRUSES
MICROORGANISMS
PARASITES
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
ULTRAVIOLET RADIATION
VIRUSES