Interferon induction by viruses. XV. Biological characteristics of interferon induction-suppressing particles of vesicular stomatitis virus

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · J. Interferon Res.; (United States)

DOI:https://doi.org/10.1089/jir.1987.7.269· OSTI ID:6368271

Marcus, P I; Sekellick, M J

A single interferon (IFN) induction-suppressing particle (ISP) of vesicular stomatitis virus (VSV) blocked completely the yield of IFN in a cell otherwise programmed to produce IFN. With mouse L cells as hosts, one lethal hit of UV radiation (D37 = 52.5 ergs/mm2) to the VSV genome sufficed to inactivate ISP activity; however, with ''aged'' primary chick embryo cells as hosts, it took 198 lethal hits (D37 = 10,395 ergs/mm2). ISP expression in chick cells did not require virus replication or amplified RNA synthesis, but did involve functional virion-associated L protein. ISP in chick cells also were capable of inhibiting, in a multiplicity-dependent manner, the plaquing efficiency of two viruses that require cellular polymerase II (pol II) for replication, e.g., pseudorabies and influenza. The refractory state to IFN inducibility that resulted from infection of chick cells with ISP (VSV tsO5 (UV = 100 hits)) was still extant after 6 days. In contrast, the plaquing efficiency of pseudorabies virus returned to control levels by 5 h after ISP infection. Chick cells infected with UV ISP remained viable, served as hosts for the replication of other viruses, and could be subcultured. Models are presented to account for these contrasting effects. The involvement of viral plus-strand leader RNA as an inhibitor of cellular pol II-dependent RNA synthesis, and the multifunctional activities of the virion-associated L protein, are discussed as possible molecules involved in the action of ISP in chick cells.

• Research Organization: Univ. of Connecticut, Storrs
• OSTI ID: 6368271
• Journal Information: J. Interferon Res.; (United States), Journal Name: J. Interferon Res.; (United States) Vol. 7:3; ISSN JIRED
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BIRDS
CELL CULTURES
CHICKENS
ELECTROMAGNETIC RADIATION
EMBRYOS
FOWL
GROWTH FACTORS
INTERFERON
LYMPHOKINES
MAMMALS
MICE
MICROORGANISMS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RNA
RODENTS
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES
VIABILITY
VIRUSES