Relationship between RNA polymerase II and efficiency of vaccinia virus replication

Journal Article · Sat Apr 01 04:00:00 EST 1989 · J. Virol.; (United States)

OSTI ID:6070181

Wilton, S; Dales, S

It is clear from previous studies that host transcriptase or RNA polymerase II (pol II) has a role in poxvirus replication. To elucidate the participation of this enzyme further, in this study the authors examined several parameters related to pol II during the cycle of vaccinia virus infection in L-strain fibroblasts, HeLa cells, and L/sub 6/H/sub 9/ rat myoblasts. Nucleocytoplasmic transposition of pol II into virus factories and virions was assessed by immunofluorescence and immunoblotting by using anti-pol II immunoglobulin G. RNA polymerase activities were compared in nuclear extracts containing cured enzyme preparations. Rates of translation into cellular or viral polypeptides were ascertained by labeling with (/sup 35/S)methionine. In L and HeLa cells, which produced vaccinia virus more abundantly, the rate of RNA polymerase and translation in controls and following infection were higher than in myoblasts. The data on synthesis and virus formation could be correlated with observations on transmigration of pol II, which was more efficient and complete in L and HeLa cells. The stimulus for pol II to leave the nucleus required the expression of both early and late viral functions. On the basis of current and past information, the authors suggest that mobilization of pol II depends on the efficiency of vaccinia virus replication and furthermore that control over vaccinia virus production by the host is related to the content or availability (or both) of pol II in different cell types.

• Research Organization: Univ. of Western Ontario, London (Canada)
• OSTI ID: 6070181
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 63:4; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL NUCLEI
CONNECTIVE TISSUE CELLS
CYTOPLASM
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EFFICIENCY
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FIBROBLASTS
FLUORESCENCE
GLOBULINS
HELA CELLS
IMMUNOGLOBULINS
INFECTIOUS DISEASES
INFECTIVITY
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
MAMMALS
METHIONINE
MICROORGANISMS
NUCLEI
NUCLEOTIDYLTRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PATHOGENESIS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RNA POLYMERASES
RODENTS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
VIRAL DISEASES
VIRUSES