Interferon induction by viruses. VIII. Vesicular stomatitis virus: (+-)DI-011 particles induce interferon in the absence of standard virions
Journal Article
·
· Virology; (United States)
Evidence was presented that VSV (+-)DI-011 particles, which contain a genome of covalently linked totally self-complementary RNA, were excellent inducers of interferon (IFN) - by virtue of the dsRNA presumed to form within an infected cell, and that one molecule of that dsRNA per cell sufficed to induce a quantum yield of IFN. While the IFN-inducing capacity of (+-)DI-011 particle preparations has been confirmed, some researchers contend that DIP by themselves cannot induce IFN and that induction requires the presence of coinfecting (contaminating) standard VSV PFP. Consequently, we reexamined this question and now report that under five different conditions where the function of contaminating standard virus is reduced markedly, or eliminated, there was no diminution of the interferon-inducing particle (IFP) activity in preparations of (+-)DI-011 particles. Thus, inactivation of contaminating PFP by uv radiation or heat, the elimination (during induction) of cycling infection through the use of anti-serum (in the case of mouse L cells), and the reduction of PFP by four successive velocity sedimentation-gradient purifications had no adverse affect on the IFN-inducing capacity of DI-011 in either ''aged'' primary chick embryo cells or in mouse L(Y) cells. Furthermore, dilutions of DI-011 stocks which precluded the presence of even a single PFP still induced IFN in ''aged'' chick embryo cells. In concert these data demonstrate convincingly that IFN induction by DI-011 particles does not require coinfection with standard virus. It follows that DI-011 particles are intrinsically capable of inducing IFN.
- Research Organization:
- Univ. of Connecticut, Storrs
- OSTI ID:
- 5658830
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 117:1; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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