Homologous interference mediated by defective interfering influenza virus derived from a temperature-sensitive mutant of influenza virus. [Ultraviolet radiation]

Journal Article · Sun Oct 01 04:00:00 EDT 1978 · J. Virol.; (United States)

OSTI ID:6858844

Nayak, D P; Tobita, K; Janda, J M; Davis, A R; De, B K

A temperature-sensitive group II mutant of influenza virus, ts-52, with a presumed defect in viral RNA synthesis, readily produced von Magnus-type defective interfering virus (DI virus) when passed serially (four times) at high multiplicity in MDBK cells. The defective virus (ts-52 DI virus) had a high hemagglutinin and a low infectivity titer, and strongly interfered with the replication of standard infectious viruses (both ts-52 and wild-type ts/sup +/) in co-infected cells. Progeny virus particles produced by co-infection of DI virus and infectious virus were also defective and also had low infectivity, high hemagglutinating activity, and a strong interfering property. Infectious viruses ts/sup +/ and ts-52 were indistinguishable from ts-52 DI viruses by sucrose velocity or density gradient analysis. Additionally, these viruses all possessed similar morphology. However, when the RNA of DI viruses was analyzed by use of polyacrylamide gels containing 6 M urea, there was a reduction in the amount of large RNA species (V1 to V4), and a number of new smaller RNA species (D1 to D6) with molecular weights ranging from 2.9 x 10/sup 5/ to 1.05 x 10/sup 5/ appeared. Since these smaller RNA species (D1 to D6) were absent in some clones of infectious viruses, but were consistently associated with DI viruses and increased during undiluted passages and during co-infection of ts-52 with DI virus, they appeared to be a characteristic of DI viruses. Additionally, the uv target size of interfering activity and infectivity of DI virus indicated that interfering activity was 40 times more resistant to uv irradiation than was infectivity, further implicating small RNA molecules in interference.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 6858844
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 28:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BIOSYNTHESIS
ELECTROMAGNETIC RADIATION
GENETICS
INFECTIVITY
INFLUENZA VIRUSES
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RNA
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES