Nongenetic complementation of group V temperature-sensitive mutants of vesicular stomatitis virus by uv-irradiated virus

Journal Article · Tue Apr 01 04:00:00 EDT 1975 · J. Virol., v. 15, no. 4, pp. 798-805

OSTI ID:4177202

Deutsch, V

Cells infected with temperature-sensitive (ts) mutants of complementation group V of vesicular stomatitis virus (VSV) give an enhanced yield at nonpermissive temperature when co-infected or superinfected with uv- irradiated virus. Virions produced in these mixed infections are temperature sensitive and do not complement ts V45. Rescue of group V mutants is multiplicity dependent. It can occur in the presence of cycloheximide; kinetics of rescue are similar in the absence or in the presence of the drug. Rescue is due to nongenetic complementation and is interpreted as a trigger effect on maturation of a small quantity of biologically active protein V molecules provided by uv-irradiated virus. These results are confirmed by rescue of ts V45 by uv-irradiated, defective, interfering T particles. (auth)

• Research Organization: Univ. of Paris, Orsay, France
• NSA Number: NSA-33-005716
• OSTI ID: 4177202
• Journal Information: J. Virol., v. 15, no. 4, pp. 798-805, Journal Name: J. Virol., v. 15, no. 4, pp. 798-805; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

*CYCLOHEXIMIDE-- BIOLOGICAL EFFECTS
*VIRUSES-- BIOLOGICAL RADIATION EFFECTS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
FIBROBLASTS
GENETICS
MUTANTS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
TEMPERATURE DEPENDENCE
ULTRAVIOLET RADIATION