Rescue at nonpermissive temperature of complementation group II temperature-sensitive mutants of vesicular stomatitis virus by uv-irradiated VSV

Journal Article · Thu Jun 01 04:00:00 EDT 1978 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(78)90162-9· OSTI ID:6736917

Deutsch, V; Brun, G

Rescue is group-characteristic. The helper virus can be either the wt strain or a mutant belonging to any group of ts mutants except group II. With regard to genotype, the rescue progeny virus is temperature-sensitive and belongs to group II, and its ts II parent (ts O52(II)) can be characterized. As for phenotype, the in vitro thermal stability of rescue virions is intermediate between that of parental ts O52(II) and uv-irradiated wt virus, suggesting incorporation of some wt protein II molecules in the rescue virions. Different slopes (zero or different from zero) were seen in dose-effect curves representing rescue obtained by structural protein molecules, suggesting that protein II structural role could be distinguished from its functional role(s) by uv sensitivity. Differences in efficiency of the rescue of ts O52(II) by ts I mutants irradiated with low uv fluence may reflect their different transcribing capabilities at 39.6/sup 0/. The results are discussed taking into account the fact that the phenotype of group II mutants is characterized by an unstable nucleocapsid.

• Research Organization: Paris Univ., Orsay, France
• OSTI ID: 6736917
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 87:1; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
GENOTYPE
MICROORGANISMS
MUTANTS
PARASITES
PHENOTYPE
RADIATION EFFECTS
RADIATIONS
TEMPERATURE EFFECTS
ULTRAVIOLET RADIATION
VIRUSES