Biological and biochemical studies on the inactivation of avian oncoviruses by ultraviolet irradiation

Journal Article · Thu Mar 31 23:00:00 EST 1977 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(77)90492-5· OSTI ID:7112570

Bister, K; Varmus, H E; Stavnezer, E; Hunter, E; Vogt, P K

The effects of ultraviolet (uv) irradiation on transforming and replicating capacities of avian oncoviruses and on the synthesis of virus specific products after infection with irradiated virus were studied. Different strains of nondefective avian sarcoma viruses were inactivated at the same rate following single-hit kinetics. The 37 percent survival dose D/sub 37/ (1/e) was 736 erg mm/sup -2/ on average. A comparison of the inactivation kinetics in a focus assay (transforming capacity) and an infectious center assay (replicating and transforming capacity) showed no partial inactivation of the virus genome; focus and infectious center formation were inactivated at the same rate. Similar results were obtained when the replicating capacity of the avian sarcoma virus was measured in a plaque assay. The rates of inactivation of avian sarcoma virus assayed in focus and infectious center tests on chick embryo fibroblasts expressing or not expressing chicken helper factor, on chick embryo cells preinfected with RAV-1, and on Peking duck cells were identical. Nondefective avian sarcoma virus and deletion mutants of avian sarcoma virus defective for replication or transformation were inactivated at the same rate. Biochemical analysis of the DNA extracted from a Japanese quail tumor cell line (QT-6) 26 hr after infection with irradiated avian sarcoma virus strain B77 showed a decrease of total virus specific DNA and of full-length covalently closed circular (form I) viral DNA synthesis with increase of the uv dose. Virus-specific RNA synthesis, measured by hybridization of labeled RNA extracted from chicken embryo fibroblasts infected with irradiated virus to viral DNA, and particle production, assayed by uridine incorporation, were also inhibited with increasing uv dose. The inactivation rates for virus-specific DNA and RNA synthesis and for particle production were very similar, but lower than the rate for the loss of infectivity.

• Research Organization: Univ. of Southern California, Los Angeles
• OSTI ID: 7112570
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 77:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
BIRDS
CELL CULTURES
CHICKENS
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
EMBRYOS
FIBROBLASTS
FOWL
HYBRIDIZATION
INACTIVATION
INJURIES
MICROORGANISMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
RNA
SOMATIC CELLS
SURVIVAL CURVES
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES