Metabolism and expression of RNA polymerase II transcripts in Influenza virus-infected cells

Journal Article · Mon Oct 01 04:00:00 EDT 1984 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.4.10.2198· OSTI ID:5823434

Katze, M G; Krug, R M

Influenza virus infection has adverse effects on the metabolism of two representative RNA polymerase II transcripts in chicken embryo fibroblasts, those coding for BETA-actin and for avian leukosis virus (ALV) proteins. Proviral ALV DNA was integrated into host cell DNA by prior infection with ALV. By S1 endonuclease assay, it was confirmed that nuclear ALV transcripts disappeared very early after infection, already decreasing ca. 80% by 1 h postinfection. A plausible explanation for this nuclear degradation is that the viral cap-dependent endonuclease in the nucleas cleaves the 5' ends of new polymerase II transcripts, rendering the resulting decapped RNAs susceptible to hydrolysis by cellular nucleases. Similar stability of cytoplasmic host cell mRNAs was observed in infected HeLa cells, in which the levels of actin mRNA and two HeLa cell mRNAs (pHe 7 and pHe 28) remained at undiminished levels for 3 h of infection and decreased only slightly by 4.5 h postinfection. The cytoplamic actin and pHe 7 mRNAs isolated from infected HeLa cells were shown to be translated in reticulocyte extracts in biro, indicating that host mRNAs were not inactivated by a virus-induced modification. Despite the continued presence of high levels of functional host cell mRNAs, host cell protein synthesis was effectively shut off by about 3 h postinfection in both chicken embryo fibroblasts and HeLa cells. These results are consistent with the establishment of an influenza virus-specific translational system that selectively translates viral and not host mRNAs.

• Research Organization: Molecular Biology and Virology Program, Graduate School of Medical Sciences, Memorial Sloan-Kettering Cancer Center, New York, NY 10021
• OSTI ID: 5823434
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:10; ISSN MCEBD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACTIN
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BIRDS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CHICKENS
CLASSIFICATION
CONNECTIVE TISSUE CELLS
DISEASES
DNA-ASE
EMBRYOS
ENDONUCLEASES
ENZYME INDUCTION
ENZYMES
ERYTHROCYTES
ESTERASES
FIBROBLASTS
FOWL
GENE REGULATION
GENES
HELA CELLS
HEMIC DISEASES
HYBRIDIZATION
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INFECTIOUS DISEASES
INFLUENZA VIRUSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LEUKEMIA
MATERIALS
MESSENGER-RNA
METABOLISM
MICROORGANISMS
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RETICULOCYTES
RNA
RNA POLYMERASES
RNA PROCESSING
SOMATIC CELLS
TRACER TECHNIQUES
TRANSCRIPTION
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES
VIRAL DISEASES
VIRUSES