Differential stability of host mRNAs in Friend erythroleukemia cells infected with herpes simplex virus type 1

Journal Article · Tue Jan 01 04:00:00 EST 1985 · J. Virol.; (United States)

OSTI ID:5833144

Mayman, B A; Nishioka, Y

The consequences of herpes simplex virus type 1 infection on cellular macromolecules were investigated in Friend erythroleukemia cells. The patterns of protein synthesis, examined by polyacrylamide gel electrophoresis, demonstrated that by 4 h postinfection the synthesis of many host proteins, with the exception of histones, was inhibited. Examination of the steady-state level of histone H3 mRNA by molecular hybridization of total RNA to a cloned mouse histone H3 complementary DNA probe demonstrated that the ratio of histone H3 mRNA to total RNA remained unchanged for the first 4 h postinfection. In contrast, the steady-state levels of globin and actin mRNAs decreased progressively at early intervals postinfection. Studies on RNA synthesis in isolated nuclei demonstrated that the transcription of the histone H3 gene was inhibited to approximately the same extent as that of actin gene. It was concluded that the stabilization of preexisting histone H3 mRNA was responsible for the persistence of H3 mRNA and histone protein synthesis in herpes simplex virus type 1-infected Friend erythroleukemia cells. The possible mechanisms influencing the differential stability of host mRNAs during the course of productive infection with herpes simplex virus type 1 are discussed.

• Research Organization: McGill Univ., Montreal, Quebec
• OSTI ID: 5833144
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 53:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTIN
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
DISEASES
DNA
DNA REPLICATION
ELECTROPHORESIS
GLOBIN
HEMIC DISEASES
HERPES SIMPLEX
HISTONES
HYBRIDIZATION
INFECTIOUS DISEASES
INFECTIVITY
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKEMIA
MAMMALS
MESSENGER-RNA
MICE
NEOPLASMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RNA
RODENTS
SKIN DISEASES
STABILITY
TRACER TECHNIQUES
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES
VIRAL DISEASES