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Gene expression of herpes simplex virus. II. Uv radiological analysis of viral transcription units

Journal Article · · J. Virol.; (United States)
OSTI ID:6538517
;
The transcriptional organization of the genome of herpes simplex virus type 1 was analyzed by measuring the sensitivity of viral polypeptide synthesis to uv irradiation of the infecting virus. Herpes simplex virus type 1 was irradiated with various doses of uv light and used to infect xeroderma pigmentosum fibroblasts. Immediate early transcription units were analyzed by having cycloheximide present throughout the period of infection, removing the drug at 8 h postinfection, and pulse-labeling proteins with (355)methionine. Delayed early transcription units were analyzed in similar studies by having 9-beta-D-arabinofuranosyladenine present during the experiment to block replication of the input irradiated genome. The results indicate that none of the immediate early genes analyzed can be cotranscribed, whereas some of the delayed early genes might be cotranscribed. No evidence was found for the existence of large, multigene transcription units.
Research Organization:
Wayne State Univ., Detroit, MI
OSTI ID:
6538517
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 34:3; ISSN JOVIA
Country of Publication:
United States
Language:
English

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Related Subjects

560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENINES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMETABOLITES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CYCLOHEXIMIDE
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EVEN-ODD NUCLEI
FUNGICIDES
GENES
HERPES SIMPLEX
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PESTICIDES
POLYPEPTIDES
PROTEINS
PURINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RNA
SKIN DISEASES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRANSCRIPTION
ULTRAVIOLET RADIATION
VIRAL DISEASES
XP CELLS