Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

RNA from an immediate early region of the type 1 herpes simplex virus genome is present in the trigeminal ganglia of latently infected mice

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
Transcription of the type 1 herpes simplex virus (HSV-1) genome in trigeminal ganglia of latently infected mice was studied using in situ hybridization. Probes representative of each temporal gene class were used to determine the regions of the genome that encode the transcripts present in latently infected cells. Probes encoding HSV-1 sequences of the five immediate early genes and representative early (thymidine kinase), early-late (major capsid protein), and late (glycoprotein C) genes were used in these experiments. Of the probes tested, only those encoding the immediate early gene product infected-cell polypeptide (ICP) 0 hybridized to RNA in latently infected tissues. Probes containing the other immediate early genes (ICP4, ICP22, ICP27, and ICP47) and the representative early, early-late, and late genes did not hybridize. Two probes covering approx. = 30% of the HSV-1 genome and encoding over 20 early and late transcripts also did not hybridize to RNA in latently infected tissues. These results, with probes spanning > 60% of the HSV-1 genome, suggest that transcription of the HSV-1 genome is restricted to one region in latently infected mouse trigeminal ganglia.
Research Organization:
Wistar Institute, Philadelphia, PA
OSTI ID:
5515078
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:10; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Expression of varicella-zoster virus and herpes simplex virus in normal human trigeminal ganglia
Journal Article · Thu Mar 31 23:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:6940854
Fine mapping of the latency-related gene of herpes simplex virus type 1: alternative splicing produces distinct latency-related RNAs containing open reading frames
Journal Article · Mon Oct 31 23:00:00 EST 1988 · J. Virol.; (United States) · OSTI ID:6046605
ICP27-dependent resistance of herpes simplex virus type 1 to leptomycin B is associated with enhanced nuclear localization of ICP4 and ICP0
Journal Article · Fri Sep 01 00:00:00 EDT 2006 · Virology · OSTI ID:20850552

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
GENES
HERPES SIMPLEX
HYBRIDIZATION
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICE
MICROORGANISMS
NERVES
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PLASMIDS
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SKIN DISEASES
SULFUR 35
SULFUR ISOTOPES
TRANSCRIPTION
VERTEBRATES
VIRAL DISEASES
VIRUSES