Expression of varicella-zoster virus and herpes simplex virus in normal human trigeminal ganglia

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.7.2362· OSTI ID:6940854

Vafai, A; Wellish, M; Devlin, M; Gilden, D H ^[1]; Murray, R S ^[2]

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1. Univ. of Colorado School of Medicine, Denver (USA)
2. Univ. of Colorado School of Medicine, Denver (USA) Veterans Administration Medical Center, Denver, CO (USA)

Lysates of radiolabeled explants from four human trigeminal ganglia were immunoprecipitated with antibodies to varicella-zoster virus (VZV) and to herpes simplex virus. Both herpes simplex virus- and VZV-specific proteins were detected in lysates of all four ganglia. Absence of reactivity in ganglion explants with monoclonal antibodies suggested that herpes simplex virus and VZV were not reactivated during the culture period. In situ hybridization studies demonstrated the presence of RNA transcripts from the VZV immediate early gene 63. This approach to the detection of herpes simplex virus and VZV expression in human ganglia should facilitate analysis of viral RNA and proteins in human sensory ganglia.

• OSTI ID: 6940854
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:7; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
GANGLIONS
LABELLING
MESSENGER-RNA
HYBRIDIZATION
VIRAL DISEASES
DIAGNOSIS
ANTIBODIES
ELECTROPHORESIS
GENES
HERPES SIMPLEX
HERPES ZOSTER
LIVER
METHIONINE
MONOCLONAL ANTIBODIES
PRECIPITATION
SULFUR 35
TRANSCRIPTION
VIRUSES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DRUGS
EVEN-ODD NUCLEI
GLANDS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROORGANISMS
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PARASITES
RADIOISOTOPES
RNA
SEPARATION PROCESSES
SKIN DISEASES
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550201* - Biochemistry- Tracer Techniques