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Fine mapping of the latency-related gene of herpes simplex virus type 1: alternative splicing produces distinct latency-related RNAs containing open reading frames

Journal Article · · J. Virol.; (United States)
OSTI ID:6046605
; ; ; ;
The latency-related (LR) gene of herpes simplex virus type 1 (HSV-1) is transcriptionally active during HSV-1 latency, producing at least two LR-RNAs. The LR gene partially overlaps the immediate-early gene ICP0 and is transcribed in the opposite direction from ICP0, producing LR-RNAs that are complementary (antisense) to ICP0 mRNA. The LR gene is thought to be involved in HSV-1 latency. The authors report here the time mapping and partial sequence analysis of this HSV-1 LR gene. /sup 32/P-labeled genomic DNA restriction fragments and synthetic oligonucleotides were used as probes for in situ hybridizations and Northern (RNA) blot hybridizations of RNA from trigeminal ganglia of rabbits latently infected with HSV-1. The two most abundant LR-RNAs appeared to share their 5' and 3' ends and to be produced by alternative splicing. These LR-RNAs were approximately 2 and 1.3 to 1.5 kilobases in length and were designated LR-RNA 1 and LF-RNA 2, respectively. LR-RNA 1 appeared to have at least one intron removed, while LR-RNA 2 appeared to have at least two introns removed. The LR-RNAs contained two potential long open reading frames, suggesting the possibility that one or more of the LR-RNAs may be a functional mRNA.
Research Organization:
Cedars-Sinai Medical Center, Los Angeles, CA (USA)
OSTI ID:
6046605
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:11; ISSN JOVIA
Country of Publication:
United States
Language:
English

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Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA SEQUENCING
GANGLIONS
GENES
GENETIC MAPPING
HERPES SIMPLEX
HYBRIDIZATION
INFECTIOUS DISEASES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MAPPING
MICROORGANISMS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RABBITS
RADIOISOTOPES
RNA
SKIN DISEASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRITIUM COMPOUNDS
VERTEBRATES
VIRAL DISEASES
VIRUSES