Herpes simplex virus types 1 and 2 induce shutoff of host protein synthesis by different mechanisms in Friend erythroleukemia cells
Herpes simplex virus type 1 (HSV-1) and HSV-2 disrupt host protein synthesis after viral infection. We have treated both viral types with agents which prevent transcription of the viral genome and used these treated viruses to infect induced Friend erythroleukemia cells. By measuring the changes in globin synthesis after infection, we have determined whether expression of the viral genome precedes the shutoff of host protein synthesis or whether the inhibitor molecule enters the cells as part of the virion. HSV-2-induced shutoff of host protein synthesis was insensitive to the effects of shortwave (254-nm) UV light and actinomycin D. Both of the treatments inhibited HSV-1-induced host protein shutoff. Likewise, treatment of HSV-1 with the cross-linking agent 4,5',8-trimethylpsoralen and longwave (360-nm) UV light prevented HSV-1 from inhibiting cellular protein synthesis. Treatment of HSV-2 with 4,5',8-trimethylpsoralen did not affect the ability of the virus to interfere with host protein synthesis, except at the highest doses of longwave UV light. It was determined that the highest longwave UV dosage damaged the HSV-2 virion as well as cross-linking the viral DNA. The results suggest that HSV-2 uses a virion-associated component to inhibit host protein synthesis and that HSV-1 requires the expression of the viral genome to cause cellular protein synthesis shutoff.
- Research Organization:
- Univ. of Colorado Health Sciences Center, Denver
- OSTI ID:
- 5978583
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 45:1; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
EXTREME ULTRAVIOLET RADIATION
HEMATOLOGIC AGENTS
HEMIC DISEASES
HERPES SIMPLEX
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
LEUKEMIA
MAMMALS
MICE
MICROORGANISMS
NEAR ULTRAVIOLET RADIATION
NEOPLASMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
SKIN DISEASES
SYNTHESIS
TRANSCRIPTION
TUMOR CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES