Ultraviolet inactivation of avian sarcoma virus: biological and biochemical analysis

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(76)90499-2· OSTI ID:7272863

Owada, M ^[1]; Ihara, S; Toyoshima, K; Kozai, Y; Sugino, Y

---

1. Osaka Univ.

The rate of inactivation by ultraviolet light of the focus-forming capacity of avian sarcoma virus was almost the same as that of the virus-producing capacity, measured as plaque formation. In addition, no significant difference was observed in inactivation of the transforming capacity assayed on C/BE chick embryo fibroblasts (CEF), which carry endogenous avian tumor virus DNA, and on duck embryo fibroblasts (DEF), which are known to be devoid of this DNA. All foci induced by nonirradiated virus produced infectious sarcoma virus, but some of the foci induced by uv-irradiated virus did not produce infectious virus of either transforming or transformation-defective type. The proportion of nonproducer foci was 3.4 times more in DEF than in gs/sup -/ chf/sup -/ CEF. RNAs extracted from uv-irradiated virions by sodium dodecyl sulfate (SDS) treatment were found to be composed of 60--70 S and 4 S RNAs by analysis in a sucrose gradient containing 0.5 percent SDS. The large RNA, however, became hydrophobic after irradiation and was sedimented with SDS by addition of one drop of saturated potassium chloride solution. This RNA was not dissociated into 30--40S components by heating at 100/sup 0/ for 45 sec, unlike 60--70 S RNA from uv-irradiated virions. After SDS--Pronase treatment, the 60--70 S RNA from uv-irradiated virions no longer had these altered characteristics. Reverse transcriptase activity with the endogenous template decreased in parallel with increase in the uv dose. The reduction rate was similar to that assayed with exogenous template or in the presence of actinomycin D. These data strongly suggest that RNA damage is not the only cause of virus inactivation by uv light.

• OSTI ID: 7272863
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 69; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIRDS
CHICKENS
CONNECTIVE TISSUE CELLS
DNA
DUCKS
ELECTROMAGNETIC RADIATION
EMBRYOS
FIBROBLASTS
FOWL
INACTIVATION
MICROORGANISMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RNA
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES