Characterization of some isolates of newly recovered avian sarcoma virus. [X Radiation]

Journal Article · Sun Dec 31 23:00:00 EST 1978 · J. Virol.; (United States)

OSTI ID:6433138

Halpern, C C; Hayward, W S; Hanafusa, H

We previously reported the isolation of a newly recovered avian sarcoma virus (rASV) from tumors of chickens injected with transformation-defective (td) mutants of the Schmidt--Ruppin strain of Rous sarcoma virus (SR--RSV). In this paper, we present further biological and biochemical characterization of the recovered sarcoma viruses. High titers of rASV's were generally obtained by cocultivation of tumor cells with normal chicken embryo fibroblasts or by homogenization of tumor tissues. Most rASV isolates were similar to SR--RSV, subgroup A (SR--RSV--A), in their growth characteristics and were nondefective in replication. The subgroup specificity of rASV's and the electrophoretic mobilities of their structural proteins were the same as those of parental td viruses. The nondefectiveness of rASV's was further substantiated by the size of their genomic RNA, which was indistinguishable from that of SR--RSV--AA and substantially larger than that of parental td RNA. Molecular hybridization using complementary DNA specific to the src gene of SR--RSV (cDNA/sub src/) showed that the RNAs of td mutants used in this study contained extensive deletions within the src gene (7 to 30% hybridization with cDNA/sub src/); the same probe hybridized up to 90% with RNA from two isolates of rASV. These data indicate that rASV has regained genetic information which had been deleted in the td mutants and strongly suggest that the generation of rASV involves a genetic interaction between td virus and host cell genetic information.

• Research Organization: Rockefeller Univ., New York, NY
• OSTI ID: 6433138
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 29:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400 -- Genetics
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BIRDS
CHEMISTRY
CHICKENS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
FIBROBLASTS
FOWL
GENETICS
HYBRIDIZATION
IONIZING RADIATIONS
MICROORGANISMS
MUTANTS
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RNA
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES
VIRUSES
X RADIATION