Tolerance induction to a thymus-dependent antigen in vitro: treatment of nonadherent cells with tolerogen biologically filtered in vitro. [Gamma radiation]

Journal Article · Sun Sep 01 04:00:00 EDT 1974 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(74)90264-0· OSTI ID:7275976

Geyer, J W; Kong, Y M

Highly tolerogenic bovine gamma globulin (BGG), a thymus-dependent antigen, was prepared by biologic filtration in vitro. It readily induced tolerance in vivo in BALB/c mice and also rendered their nonadherent lymph node cells tolerant after in vitro incubation. Biologic filtration in vitro was carried out by incubating 2.5 x 10/sup 7/ lymph node cells with 10 mg of nontolerogenic BGG in 10 ml of Eagle's medium containing 2 percent normal mouse serum at 37/sup 0/C for 6 hr. The BGG-containing medium was clarified by centrifugation and was used without further dilution. For tolerance induction in vitro, lymph node cells were separated into adherent and nonadherent populations on Falcon plastic. These cells were incubated for 0 to 18 hr at 37/sup 0/C with biologically filtered BGG (bBGG). After incubation, the cells were washed three times and (2 to 2.5) x 10/sup 7/ nonadherent or 4 x 10/sup 6/ adherent cells were injected iv with their untreated counterpart into lethally irradiated mice which had received 10/sup 6/ bone marrow cells. The recipients were then challenged with 300 ..mu..g of aggregated BGG, and tolerance was assayed by the elimination of labeled BGG, rosette formation, and passive hemagglutination. Spleen cells were similarly treated for comparison. Our findings show that tolerance was not induced in vitro in adherent lymph node cells. However, in the nonadherent populations, those from the lymph node but not the spleen were rendered tolerant. The acquisition of tolerance in vitro was gradual. It was dependent upon the length of exposure to bBGG and required at least 6 hr.

• Research Organization: Wayne State Univ., Detroit
• OSTI ID: 7275976
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 13:3; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GLOBULINS
GLOBULINS-GAMMA
IMMUNE REACTIONS
IONIZING RADIATIONS
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MICE
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
THYMUS
VERTEBRATES